Proteoform Thursday: Fabio Gomes presents “Native Top-Down Characterization of Proteoforms and their Complexoforms in Breast Cancer Cells”
July 25, 2024 11:00 AM in Eastern Time (US and Canada)
Oligomerization of proteins and their modified forms (proteoforms) produces functional protein complexes. Complexoforms are complexes that consist of the same set of proteins with different proteoforms. The ability to characterize these assemblies within cells is critical to understanding the molecular mechanisms involved in disease and to designing effective drugs. I will present a native top-down proteomics (nTDP) strategy that combines size-exclusion chromatography, nano direct infusion, field asymmetric ion mobility spectrometry, and multistage mass spectrometry to identify protein assemblies (≤70 kDa) in breast cancer cells and in cells that overexpress EGFR, which serves as a resistance model of estrogen receptor-α (ER-α) targeted therapies.