Top Down ETD Antibody Analysis

Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry.


Dnph Carbonyl Essay Biomolecular Mass Spectrometry Laboratory, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland. This email address is being protected from spambots. You need JavaScript enabled to view it.

• Used state of the art ETD-MS/MS instrument (maXis UHR qTOF MS, by Bruker Daltonics)
Characterized two intact recombinant monoclonal antibodies, murine and human IgGs.
The top down ETD data for fragmentation of intact antibodies is superior compared to CID performed on the entire IgG.