Kelleher Lab IDs 3,000-Plus Protein Species with New High-Throughput Top-Down Proteomics Platform

A team of scientists led by Northwestern University researcher Neil Kelleher has developed a workflow for high-throughput top-down proteomics, using it to identify more than 3,000 protein species in an analysis of HeLa S3 cells.

According to Kelleher, this number of IDs represents a 20-fold increase over any previous top-down experiments in mammalian cells and is one of the first demonstrations of the feasibility of large-scale, discovery-style intact protein work.