Formative Meeting of the Consortium for Top Down Proteomics

Formative Meeting of the Consortium for Top Down Proteomics

A consortium for top-down proteomics "CTDP" met in early March 2012 in Lisbon, Portugal to discuss the current status and future prospects of top-down proteomics. The consortium was formed in the hopes of promoting innovative research, collaboration and education to accelerate the comprehensive analysis of intact proteins in complex systems.

http://www.meandergroep.com/?master-thesis-review-services Master Thesis Review Services One of the goals of the consortium is to establish standardized methods, databases, and terminology such that we can create a universal language of top-down proteomics. At this time, top-down proteomics is an expensive technology. However, as we've seen in the past with the Human Genome Project, a relatively small stimulus can have large effects and will make proteomic analysis cheaper and more effective.

We believe that top-down proteomics will fill a much needed gap that cannot be accomplished by bottom-up techniques. By filling in these gaps, we hope to identify new proteins and pave a pathway for a human proteome project.

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2013 Sanibel Conference on Top Down Mass Spectrometry

 2013 Sanibel Conference on Top Down Mass Spectrometry

Co-Organizers: Neil L Kelleher, Northwestern University and Ljiljana Paša-Tolić, PNNL Top Down mass spectrometry is a classical approach to molecular analysis, but is reborn in the contemporary context of protein analysis. Top Down now stands at a turning point – somewhat beyond an emerging technology, but yet difficult to implement vs. more widely used Bottom Up approaches to protein and proteome interrogation. For proteomics, Top Down strives to preserve the forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis, with the largest impact thus far felt in chromatin biology via Top Down and Middle Down analysis of histones. For single biopharmaceutical targets that are highly modified, Top Down continues its acceleration toward an accepted, even wide-spread practice. This meeting will bring together novice and seasoned practitioners in the field of Top Down mass spectrometry with the goal to outline recent approaches and applications and provide an outlook for its future.

The conference will be held January 24 - 27, 2013. For more information, please see the ASMS website.

10th Uppsala Conference (UppCon) on ECD/ETD and Related Topics

10th Uppsala Conference (UppCon) on ECD/ETD and Related Topics

The 10th Uppsala Conference (UppCon) on ECD/ETD and related topics will be held February 16-21, 2013 in Beijing, China. Organizers of the conference are Meng-Qiu Dong of the National Institute of Biological Sciences (NIBS) in Beijing and Rui-Xiang Sun of the Institute of Computing Technology, Chinese Academy of Sciences

The topics of the Uppsala Conference encompass theories, experiments, instrumentation, and applications related to ion-ion or ion-electron reactions. UppCon rotates among North America, Europe, and Asia. This is the first time that UppCon comes to Mainland China. A training course is designed to introduce more students and researchers to fundamental mass spectrometry.

For more information on the program and how to register, please see the UppCon 2013 website.

Chemical and Engineering News Cover Story

Top-Down Proteomics Becomes Reality

Chemical and Engineering News Cover Story

Chemical and Engineering NewsIn the May 20, 2013 issue of C&E News (Volume 91, Issue 20), Senior Editor, Celia Henry Arnaud profiles the field of Top Down Proteomics in a cover story titled: "Top Down Proteomics Becomes Reality."


Cv Writing Service Us In the mid-1990s, as the Human Genome Project was in full swing, scientists started thinking about the protein complement of the genome, and proteomics—the identification and characterization of all of an organism’s proteins—was born. Early proteomics methods used enzymes to digest proteins into pieces that could be easily analyzed by mass spectrometry. Those methods are now mature and routinely detect peptides from thousands of proteins in a single run.

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61st ASMS Conference on Mass Spectrometry and Allied Topics

Minneapolis Convention Center, Minneapolis, MN

61st ASMS Conference on Mass Spectrometry and Allied Topics

The 61st ASMS Conference on Mass Spectrometry and Allied Topics will be held June 9-13, 2013 in Minneapolis, MN. 

The conference program begins on Sunday, June 9 with tutorial lectures at 5:00 pm and the opening session and plenary lecture at 6:45 pm. A Welcome Reception in the poster/exhibit hall will follow. Monday through Thursday are full program days of concurrent oral sessions, poster sessions, and workshops. Poster sessions are scheduled 10:30 am to 2:30 pm to allow four hours of viewing. The program will conclude on Thursday, June 13 with a plenary lecture followed by a Closing Gala at the Convention Center (ticket required).

The Consortium for Top Down Proteomics will be meeting on Monday, June 10, 2013 at a workshop from 5:45pm - 7:00pm.  Organizers will be Ying Ge from the University of Wisconsin and Nick Young from the National High Magnetic Field Laboratory.  We will see you then!

For more information on the program and how to register, please see the ASMS website.

 

  Writing Helps Top Down Oral Presentations

Session Time Room Speaker Title
MOH-am 9:30 103 K. Breuker Characterization of Modified RNA by Top Down Mass Spectrometry
MOB-pm 2:30 L100 M. Gross Fragmenting Intact Macromolecules and Protein Assemblies: Native ESI and Top-down MS for Protein Biophysics and Analysis
MOB-pm 2:50 L100 Y. Ge Top-down Mass Spectrometry Enabled Cardiac Proteomics for Understanding Heart Failure
MOB-pm 3:10 L100 A. Marshall Unequivocal Determination of Site-Specific Protein Disulfide Bond Reduction Potentials by Top-Down FT-ICR MS/MS
MOB-pm 3:30 L100 B. Ganisl Detailed characterization of complex protein and RNA modification patterns by top-down mass spectrometry
MOB-pm 3:50 L100 L. Fornelli Antibody characterization by top-down and middle-down electron transfer dissociation using a high-field Orbitrap FTMS
MOB-pm 4:10 L100 A. Catherman Comprehensive Top Down Proteomics of Human Cells: The Role of Mitochondria and Membrane Proteins in Cellular Senescence
TOH-am 8:30 103 L. Dekker An integrated top-down and bottom-up proteomic approach to characterize antibodies
TOH-am 8:50 103 W. Jabs In-depth Mass Spectrometry Characterization of Therapeutic Antibodies for Efficient Biosimilar Development
TOB-am 9:10 L100 A. Kiss Mass Spectrometry Imaging with a LAESI hybrid iontrap FT-ICR mass spectrometer
TOH-am 9:30 103 W. Wang An Online Top-down Mass Spectrometry Based Strategy Aimed at Complete De novo Sequencing of Monoclonal Antibodies
TOH-am 9:50 103 S. Hengel Micro-Scale Native Top-Down LCMS of Cysteine-Linked Antibody-Drug Conjugates
TOH-pm 2:50 103 M. ElNaggar Applications of an in situ Microextraction Based Surface Sampling System to Microorganism Analysis
TOA-pm 4:10 Ex Hall A J. Shaw Intact Protein Characterization by 193 nm Ultraviolet Photodissociation in an Orbitrap Elite
WOE-am 8:30 Aud. E. Nikolaev The way to isotopic resolution of mega Dalton protein mass spectra for top-down proteomics
WOD-pm 3:30 Ball A J. Savaryn Quantifying Proteoforms using High-Throughput Top-Down Proteomics for Cell-Based Biomarker Discovery
WOB-pm 3:50 L100 R. LeDuc Using Prior Knowledge to Improve Scoring in High-Throughput Top-Down Proteomics Experiments
ThOA-am 9:10 Ex Hall A C.C. Hsu  Microscopy Guided Atmospheric Ionization In Situ Top-Down Protein Mass Spectrometry
ThOB-pm 3:50 L100 J. Chamot-Rooke Exploring Neisseria meningitidis Virulence with Top-Down Mass Spectrometry

An all-encompassing term to describe protein complexity

Neil Kelleher and Lloyd Smith propose that the scientific community adopt the term ‘proteoform’ to refer to all the different forms that a protein can take. Will the community adopt it?

An all-encompassing term to describe protein complexity

In the March 2013 issue of Nature Methods, Neil Kelleher and Lloyd Smith, investigators within the Consortium for Top Down Protoemics, have proposed a new term for the myriad forms of proteins that can be found in nature.

See the Methagora blog post by Allison DoerrAn all-encompassing term to describe protein complexity

Read the Article in Nature Methods: Proteoform: a single term describing protein complexity

US HUPO: Frontiers in Proteomics

Advancing Biology through Technology and Computation

US HUPO: Frontiers in Proteomics
We hope you will all join us in Seattle for the 10th Annual Conference. As the Society continues to mature US HUPO sharpens its focus on the next generation of researchers with the establishment of the Robert J. Cotter New Investigator Award in 2013 and increased support for student and post-doc travel stipends. The annual meeting provides opportunities to experience presentations from top scientists, both up-and-coming and established, on cutting-edge topics.

62nd ASMS Conference on Mass Spectrometry and Allied Topics

Baltimore Convention Center, Baltimore, MD

62nd ASMS Conference on Mass Spectrometry and Allied Topics

The 62nd ASMS Conference on Mass Spectrometry and Allied Topics will be held June 15-19, 2014 in Baltimore, MD. 

The conference program begins on Sunday, June 15 with tutorial lectures at 5:00 pm and the opening session and plenary lecture at 6:45 pm. A Welcome Reception in the poster/exhibit hall will follow. Monday through Thursday are full program days of concurrent oral sessions, poster sessions, and workshops. Poster sessions are scheduled 10:30 am to 2:30 pm to allow four hours of viewing. The program will conclude on Thursday, June 19 with a plenary lecture followed by a Closing Gala at the Convention Center (ticket required).

For more information on the program and how to register, please see the ASMS website.

 

  Top Down Oral Presentations (TBD)

Publish your Dataset (for free!) with the CTDP

Publish your Dataset (for free!) with the CTDP

As many of you know, earlier this year, we rolled out the ability to publish datasets with the Consortium for Top Down Proteomics (CTDP).  In a collaboration with the Indiana University "IU ScholarWorks" Repository, the CTDP is able to publish data for long-term storage.  If you are interested, please contact the CTDP Dataset Manager, This email address is being protected from spambots. You need JavaScript enabled to view it.

Once published, you will have a stable "Uniform Resource Identifier" to include with your journal publication and we will link to it from the Consortium's Datasets Page, http://topdownproteomics.org/data. An example of a published dataset can be found here: http://topdownproteomics.org/data/item/ultraviolet-photodissociation-for-characterization-of-whole-proteins-on-a-chromatographic-time-scale

Share your Research with the CTDP Community

Share your Research with the CTDP Community

We'd like to solicit your help in promoting your research.  If you'd like your top-down research promoted on the sliding images above, please create a slide for us and we'd be happy to feature it on the site.  

If you're comfortable making an image file, please follow the guidelines in the image shown here for general locations of information.  The final size of the image in the slideshow is 650 x 300 px, PNG image format preferred.  We often scale down 1730 x 800 px to ensure that there's enough resolution.  

If you'd prefer a powerpoint template, please see the file linked below (Note that the size of the template slide is 18" x 8.33" not the traditional PowerPoint size.  Once you're done, please send that image (or powerpoint file) to the Consortium's webmaster @ This email address is being protected from spambots. You need JavaScript enabled to view it..  If the quality needs adjustment, we'll work with you.